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Para-aminohippuric acid (PAH), an indicator of renal plasma flow, is a commonly usedmarker of organic anion transport by the kidney. An analytical method for PAH using HPLC was developed. The method is simple, fast and requires a minimum amount of organic solvent. Sample preparation involved protein precipitation with zinc sulfate.

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  • How is para-aminohippuric acid measured?

  • Because the concentration of para-aminohippuric acid in peripheral veins is equal to the concentration in the renal artery, this can be easily sampled along with the clearance of it into the urine in order to estimate renal plasma flow. Renal blood flow is then calculated by dividing renal plasma flow by 1 minus hematocrit.

  • What is renal plasma flow (RPF)?

  • Which in turn leads to: Renal Plasma Flow (RPF) = [PAH] in urine x Urine flow rate / [PAH] in plasma Urine flow rate V 鈥?ml/min or cc/min.

  • What is the formula for renal plasma flow in urine?

  • RPF x [PAH] in plasma = [PAH] in urine x Urine flow rate. Which in turn leads to: Renal Plasma Flow (RPF) = [PAH] in urine x Urine flow rate / [PAH] in plasma. With: Renal Plasma Flow – ml/min or cc/min; [PAH] in urine or in plasma 鈥?in mg/ml;

  • What is the relationship between PAH and renal venous blood?

  • Para amino hippuric acid, or PAH, is avidly secreted by the renal tubules so that nearly all of the blood that enters the kidneys is 鈥渃leared鈥?of PAH and renal venous blood contains very little PAH. The amount of PAH appearing in the urine per unit time, then, is equal to the amount contained in the blood that perfuses the kidney per unit time.